|
KMID : 0352720080320020114
|
|
Journal of Ginseng Research
2008 Volume.32 No. 2 p.114 ~ p.119
|
|
|
Isolation and Characterization of Terpene Synthase Gene from Panax ginseng
|
|
Kim Yu-Jin
Ham Ah-Rom
Shim Ju-Sun
Lee Chung-Hyae
Jung Dae-Young
In Jun-Gyo
Lee Bum-Soo
Yang Deok-Chun
|
|
|
Abstract
|
|
|
|
Terpene synthase plays a key role in biosynthesis of triterpene saponins (ginsenosides) and is intermediate in the biosynthesis of a number of secondary metabolites. A terpene synthase (PgTPS) cDNA was isolated and characterized from the root of Panax ginseng c.A. Meyer. The deduced amino acid sequence of PgTPS showed a similarity with A. deliciosa (AAX16121) 61%, V. vinifera (AAS66357) 61%, L. hirsutum (AAG41891) 55%, M. truncatula (AAV36464) 52%. And the segment of a terpene synthase gene was amplified by reverse transcriptase-polymerase chain reaction (RTPCR). We studied expression of terpene synthase under stressful conditions like chilling, salt, UV, and heavy metal stress treatment. Expression of PgTPS was increased gradually after exposure to stresses such as chilling, salt, and UV illumination. But its transcription seems to be reduced by cadmium and copper treatment.
|
|
|
KEYWORD
|
|
|
Panax ginseng, terpene synthase, abiotic stress, cDNA, RT-PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|